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| NUCLEOGEL® RP |
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polymer-based reversed phase HPLC phases
for biochemical applications
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Features
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polystyrene resin cross-linked with divinylbenzene, available particle sizes 5 μm and 8 μm,
available pore sizes 100 Å, 300 Å, 1000 Å and 4000 Å ∙ USP L21
pH working range 1 – 13, max. working pressure 180 bar
eluent in column acetonitrile / water
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small pore columns for reversed phase separation of small molecules such as pharmaceuticals with basic properties, e. g. organic heterocycles
also suited for separation of nucleosides and nucleotides up to 5000 daltons
allow gradient as well as isocratic elution
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wide pore columns are especially recommended for large biomolecules (proteins, peptides etc.)
higher background hydrophobicity compared to silica phases
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Ordering information
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Length
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50 mm
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150 mm
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250 mm
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300 mm
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Guard columns*
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| NUCLEOGEL® RP 100-5 / RP 100-8 (pore size 100 Å) |
| Valco type analytical columns |
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4.6 mm ID |
5 µm particles
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719454
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719455
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719542
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| 4.6 mm ID |
8 µm particles
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719456
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719520
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719542 |
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| 7.7 mm ID |
8 µm particles |
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719457
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719542 |
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| NUCLEOGEL® RP 300-5 / RP 300-8 (pore size 300 Å) |
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| Valco type analytical columns
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4.6 mm ID |
5 µm particles
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719459
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719542 |
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| 4.6 mm ID |
8 µm particles
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719460
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719542 |
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| 7.7 mm ID |
8 µm particles
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719463
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719542 |
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| NUCLEOGEL® RP 1000-8 (pore size 1000 Å) |
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| Valco type analytical columns |
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4.6 mm ID |
8 µm particles
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719461
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719510
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719542 |
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| 7.7 mm ID |
8 µm particles |
719464
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719542 |
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| NUCLEOGEL® RP 4000-8 (pore size 4000 Å)
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| Valco type analytical columns |
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4.6 mm ID |
8 µm particles
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719462
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719542 |
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| 7.7 mm ID |
8 µm particles |
719465
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719542 |
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| * Valco type guard column cartridges measure 5 x 3 mm, require guard
column holder B (Cat. No. 719539) and are supplied in packs of 2.
Columns are supplied in packs of 1.
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