CHROMABOND® XTR
kieselguhr phase for liquid-liquid extraction
Features

	base material coarse-grained kieselguhr (also known as diatomaceous earth, hydromatrix, celite) large pore size, high pore volume, constantly high batch-to-batch quality pH working range 1 – 13
	application: liquid-liquid extraction of highly viscous aqueous solutions such as physiological fluids (blood, plasma, and serum) in clinical chemistry, dyes in textiles, environmental and food analysis without use of a separation funnel high water loadability without breakthrough of water during elution with organic solvents also suited for removing small amounts of water from solvents which are not miscible with water
	advantages: fast, reproducible and economical simultaneous preparation of several samples no problems with phase separation ∙ no formation of emulsions high recovery rates saving of time and solvents organic solutions need not to be dried after separation

General column parameters						Solvents applicable for elution
CHROMABOND XTR		max. volume capacity of aq. solution	waiting period before elution	elution volume		diethyl ether tert-butyl methyl ether ethyl acetate n-hexane cyclohexane toluene dichloromethane (methylene chloride) trichloromethane (chloroform) solvent mixtures: trichloromethane / methanol (90:10, v/v) trichloromethane / methanol (85:15, v/v) diethyl ether / ethanol (90:10, v/v) diethyl ether / ethanol (80:20, v/v) dichloromethane / 2-propanol (90:10, v/v) dichloromethane / 2-propanol (85:15, v/v)
volume	amount of adsorbent
1 ml	250 mg	0.25 ml	5 min	3 ml
3 ml	500 mg	0.5 ml	5 min	6 ml
6 ml	1 g	1 ml	5 - 10 min	8 ml
15 ml	3 g	3 ml	5 - 10 min	12 ml
30 ml	4.5 g	5 ml	5 - 10 min	16 ml
45 ml	8.3 g	10 ml	10 - 15 min	24 ml
70 ml	14.5 g	20 ml	10 - 15 min	40 ml
150 ml	37.5 g	50 ml	10 - 15 min	90 ml
Depending on the concentration of the analytes eluates can be analysed immediately, or the organic solvent is evaporated. The pH value of the aqueous solution can be altered on the column, which enables elution of different compounds of a sample under optimised conditions. Under certain circumstances, acidic, neutral, and basic compounds can be fractionated in this way.						Eluents with too high alcohol contents cause an increase in volume of the aqueous phase on the CHROMABOND XTR. Here the column could be overloaded and the aqueous phase displaced from the column. In this case, a greater capacity column should be used.

Ordering information

	column volume	1 ml	3 ml	6 ml	15 ml	30 ml	45 ml	70 ml	150 ml
	adsorbent weight	250 mg	500 mg	1 g	3 g	4.5 g	8.3 g	14.5 g	37.5 g
	pack of	100	50	30	30	30	30	30	10
	CHROMABOND® XTR polypropylene columns
		730501	730502	730487	730489	730505	730506	730507	730509
	CHROMABOND® XTR polypropylene columns · BigPacks
			730487.250 (pack of 250)				730507.100 (pack of 100)
	CHROMABOND® MULTI 96 XTR
	96er plates 96 x 150 mg, packs of 1 plate, for max. 96 x 0.2 ml aqueous solution
			738131.150M
	CHROMABOND® XTR adsorbent
	50 bags of 14.5 g (for max. 20 ml aqueous solution each)		for 70 ml PP columns with 100 PE filter elements					730585
			for NT20 with 50 PE filter elements (dia. 10 mm)					730586
					500 g			730595.500
					1 kg			730595.1000
					5 kg			730595.5000
	Accessories for liquid-liquid extraction with CHROMABOND® XTR
	variable polypropylene rack for 24 positions, incl. 24 PP stopcocks and 24 PP needles								730508
Glass columns on request