porablot NCP

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porablot NCP/NCL

porablot NCP
Nitrocellulose membranes

Features

The classical transfer medium for binding proteins and nucleic acids

Membrane material 100 % nitrocellulose

Membrane character Hydrophilic

Pore size
0.45 µm

Binding capacity
100 µg/cm²

Applications

• Southern, Northern, and Western blotting, colony and plaque transfer, dot blotting
• Also available with a supporting tissue, see porablot NCL

Membrane filters of high purity nitrocellulose are the classical transfer medium for binding proteins and nucleic acids. The range of application includes Southern, Northern and Western blotting as well as colony, plaque transfer and dot blotting. Although the binding mechanism is not understood in all details, it is assumed that the macromolecules are bound via hydrophobic interaction and hydrogen bonding. porablot NCP membranes consist of 100% nitrocellulose and are supplied with a pore size of 0.45 μm. With a binding capacity of 100 μg/cm² this membrane is well suited for protein blotting. The hydrophilic character of this membrane ensures good contact with the gel during botting. Additionally, nitrocellulose can be easily blocked, e.g. with casein or albumin. Due to the low background, all conventional detection methods, such as antigen/antibody, autoradiography, biotin labelling and chemiluminescence, yield good results.

Ordering information

Product
Size

Pack of
Pore size [µm] Reference

porablot NCP
0.3 x 3 m

1 roll
0.45 741280

200 x 200 mm
10 sheets 0.45 741281

Ø 132 mm
50 disks 0.45 741282

Ø 82 mm
50 disks 0.45 741283

For ordering click here